Approved on: | 2024-11-01 |
Brief purpose of the study: | The purpose of this research study is to look at how your skeletal muscles, liver, and fat tissue react to hormones that normally control your blood glucose (sugar). We will evaluate body composition, insulin sensitivity, and how your cells in your adipose tissue (fat) work to regulate blood sugar. |
Brief summary of what participants will be asked to do: | All procedures are for research purposes only and will be performed at the University of Missouri in Columbia. Visits will be conducted in the Clinical and Translational Science Unit (CTSU) and the NextGen Clinical and Translational Imaging Unit (CTIU). There will be three cohorts: lean, obese, and modified obese. Participants will be compensated for their study involvement.
Overview of this study
• Screening Visit 1: You will be asked to complete the screening evaluation after you have given informed consent to determine your eligibility for this study. The research team will discuss the inclusion and exclusion criteria with you during the consenting and medical screening process. Only people who meet the eligibility criteria from the tests performed during the screening visit will be asked to continue.
• Visit 2 (Body composition): Your body fat and lean mass will be determined by using a dual energy X-ray (DEXA) machine. In addition, your liver fat content and abdominal adipose tissue mass (fat in your belly) will be determined by magnetic resonance imaging/spectroscopy (MRI/MRS). Only people who meet the eligibility criteria from the tests performed during the screening visit will be asked to continue.
• Visit 3 (Infusion visit: glucose/insulin infusion with stable blood sugar and muscle and fat [adipose] tissue biopsies): You will complete a study visit that includes an overnight stay in the clinical research unit and lasts about 24 hours. This is sometimes called a “clamp visit,” which refers to a scientific description of the glucose-insulin infusion.
[Obese participants not in modified obese group only]:
• Weight loss intervention: After completing Visit 2 and Visit 3, you will be asked to complete a weight loss intervention to help you achieve a 10% body weight loss. You will meet with a member of the study team to help you lose weight by providing guidance on what you eat and how to curb your hunger. Your body weight will also be measured. You will meet with the study team member at regular intervals (about biweekly). You will be asked to not start an exercise program while losing weight.
• Repeat Testing: Once you have completed the weight loss intervention, Visit 2 (Body composition) and Visit 3 (Infusion) will be repeated after you have lost about 10% of your body weight (about 6 months after starting the weight loss intervention). |
Basic eligibility criteria: | Between 18-70 years old; not pregnant or breastfeeding; weight stable and sedentary before enrollment; no use tobacco products, excessive amounts of alcohol, or dietary supplements, or medications known to or suspected to affect glucose and lipid metabolism; and no evidence of significant organ system dysfunction or disease (e.g. chronic severe kidney disease, cancer). |
Study contact for more information: | MittendorferLab@umsystem.edu |
IRB Number: | 2098077 MU |